F200 / M200

Life Science

Detail

Infinite® 200 PRO: detection solutions that grow with your ideas

Immediate access to all wavelengths in an affordable, scaleable detection family –

with the patent pending NanoQuant Plate™ and Gas Control Module (GCM™)

Infinite 200 PRO – New horizons in microplate detection

Building on the success of the Infinite 200 series, Tecan has developed the

Infinite 200 PRO, with new enhancements that cater to the needs of today’s

scientists. The Infinite 200 PRO offers flexible, scaleable detection solutions

for a wide range of assays, using monochromator- and filter-based technologies.

 

Specification:

Light source

UV Xenon flashlamp

     
         

Wavelength selection

       

Infinite M200 PRO

Quad4 Monochromators system (2 excitation and 2 emission monochromators)

Bandwidth Ex:

< 5 nm for λ ≤ 315 nm and < 9 nm for λ > 315 nm; Em: < 20 nm

 

Absorbance

 Fluorescence

Wavelength accuracy

 < ± 0,5 nm for λ > 315 nm; < ± 0,3 nm for λ ≤ 315 nm

< ± 2 nm for λ > 315 nm; < ± 1 nm for λ ≤ 315 nm

Wavelength reproducibility

< ± 1 nm for λ > 315 nm;

 < ± 0,5 nm for λ ≤ 315 nm

Infinite F200 PRO

 Up to 4 filter pairs per slide

         

Wavelength range

       

Fluorescence intensity

Standard: Ex 230 – 600 nm, Em 330 – 600 nm
Optional: Ex 230 – 850 nm, Em 280 – 850 nm

Fluorescence polarization

Standard: Ex 300 – 600 nm; Em 330 – 600 nm
Optional: Em 330 – 850 nm

Absorbance

 230 – 1000 nm

     

Detectors

Fluorescence – PMT, optional UV and red-sensitive

 
 

Absorbance – UV silicon photodiode

   
 

Luminescence – photon counting system with low dark current PMT

 

Plate formats

6- to 384-well plates, cuvettes, NanoQuant Plate

 

Temperature control

Ambient +5 °C up to 42 °C

   

Shaking

Linear, orbital

     
         

Fluorescence sensitivity* values

Infinite F200 PRO

 

Infinite M200 PRO

 

Fluorescence top reading*

85 amol / well (0,85 pM, 384-well plate)

 170 amol / well (1,7 pM; 384-well plate)

Fluorescence bottom reading*

0,7 fmol / well (3,5 pM; 96-well plate)

 1,2 fmol / well (6 pM; 96-well plate)

TRF**

2,8 amol / well (28 fM; 384-well plate)

90 amol / well (0,9 pM; 384-well plate)

FP*

< 4 mP standard deviation @ 1 nM Fluorescein

N / A

 

Luminescence sensitivity values

 Standard

     

Glow luminescence***

225 amol ATP / well (9 pM; low volume 384-well plate)

 

Flash luminescence****

12 amol ATP / well (218 fM; 384-well plate)

   

 

       

Absorbance

       

Ratio accuracy 260 / 280 nm

± 0,07

     

Precision @ 260 nm

 < 0,2 %

     

Accuracy @ 260 nm

 < 0,5 %

     

Measurement range

 0 – 4 OD

     

Injectors

       

Pump speed

 100 – 300 μl/s

     

Injection volume

selectable in 1 μl increments; max. volume: 800 μl per stroke

 

Dead volume

100 μl including pump back

   

Fastest Read Times

       

96 well plate

 20 sec

     

384 well plate

 30 sec

     

Wavelength Ex / Em-scan, 96 well plate

     

450 – 550 nm, 5 nm step

 150 sec

     

Source:  brochure

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